MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy
The non-destructive collection of ultrathin sections on silicon wafers for post-embedding staining and volumetric correlative Womens Sweaters light and electron microscopy traditionally requires exquisite manual skills and is tedious and unreliable.In MagC introduced here, ORG PUMPKIN sample blocks are augmented with a magnetic resin enabling the remote actuation and collection of hundreds of sections on wafer.MagC allowed the correlative visualization of neuroanatomical tracers within their ultrastructural volumetric electron microscopy context.